The gene that cracked the cancer code by sue armstrong. A c et y lat ion of p53 is mediat ed by p300 and cb p ac et y lt rans f eras es. Zebrafish danio rerio, a minnowlike freshwater fish, has emerged as a popular animal model for research and development. Antip53 antibody products listed below are antip53 antibodies from multiple suppliers.
Pdf smc3 knockdown triggers genomic instability and p53. P53 antibody rabbit polyclonal from proteintech validated in western blot wb, immunoprecipitation ip, immunofluorescence if, coimmunoprecipitation ip, chimmunoprecipitation ip, enzymelinked immunosorbent assay elisa applications. Zebrafish antibodies primary antibodies sigmaaldrich. It is not a book that will tell you the detailed molecular mechanism by which this gene will prevent or cause cancer. The protein encoded by this gene is a member of the stat family of transcription factors.
However, any fluorophoreconjugated antibody with a userpreferable absorptionemission spectra can be used here. Genetic modeling of lifraumeni syndrome in zebrafish disease. Activation of p53 leads to induction of dna repair, cellcycle arrest, apoptosis and. Anti p53 antibody products listed below are anti p53 antibodies from multiple suppliers. Please use this form to recommend updates to the information in zfin. Western blot analysis of cell extracts using p53 antibody. Through the personal accounts of key researchers, the book reveals the excitement of the hunt for new curesthe hype, the enthusiasm, the.
The completion of the human genome sequence international human genome sequence consortium 2001nature 409, 860921. Oct, 2019 results demonstrate that zebrafish p53 protein can readily be induced in embryos and detected using these new antibody tools, which will increase the usefulness of zebrafish as a model in compoundbased screening for novel drugs in cancer research. Antibodyplus provides trial size antibody samples for antibody validation. A 53 kda band corresponding to p53 was observed across cell lines tested except skov3. Genetic modeling of lifraumeni syndrome in zebrafish. For example, the fulllength amino acid sequence of zebrafish p53 is 48. Not surprisingly, p53 is the most studied single gene in history.
We have, therefore, generated a panel of antizebrafish p53 monoclonal antibodies and. Anti p53 antibody a 1 is recommended for detection of p53 of mouse, rat and human origin by wb, ip, if and elisa. Replacement to abcam, santa cruz, sigma and cst antibody. We investigated the chronic effects of deltamethrin on gonads by histopathological examination. A guide for the laboratory use of zebrafish danio rerio. Furthermore, as with some lfs mutations, the zebrafish p53i166t allele is a. Our results demonstrate that zebrafish p53 protein can readily be induced in embryos and detected using these new antibody tools, which will increase the usefulness of zebrafish as a model in. Loss of p53 response is associated with cell proliferation and invasion, and mutations in the p53 gene are found in over 50% of all human cancers. Mitotic cells in stac mutants show chromosome missegregation and are usually arrested in g2m phase. Rabbit polyclonal antip53 mono methyl lys370 antibody stj97694. Zebrafish have a competent p53dependent nucleotide. Zfin provides a wide array of expertly curated, organized and cross. Such mutations probably facilitate carcinogenesis primarily through abrogating the tumor suppressor activities of the wild type p53 protein, although. Rabbit monoclonal y5 to mutant p53 bsa and azide free.
Analysis of apoptosis in zebrafish embryos by wholemount. The p53 promoter polymorphism is not associated with the susceptibility of uterine leiomyomas in iranian women. This antibody reacts with human, rat, mouse samples. Applications key ab array antibody array ae affinity electrophoresis affin chroma. Loss of p53 synthesis in zebrafish tumors with ribosomal protein. Phosphop53 ser15 antibody 9284s from cell signaling. The zebrafish danio rerio is a freshwater fish belonging to the minnow family of the order cypriniformes. Hrpcoupled antimouse and antirabbit antibodies dako, glostrup, denmark. Recent developments in p53 antibody research p53 is a stressactivated transcription factor, encoded by the tp53 gene. Due to their striking genomic similarity to humans, regenerative abilities, and shortened life cycle, zebrafish have become an ideal tool for studying developmental processes, gene expression, and underlying mechanisms of cancer and other diseases. An important tumor suppressor, the protein mediates cellular growth and proliferation. After uv dna damage, the tumor suppressor p53 plays a critical role in halting the cell cycle to allow for repair of the damage, or initiating cell death. Hardcopies of the 4th edition of the zebrafish book can be obtained for a nominal fee from the zebrafish international resource center, 5274 university of oregon, eugene, or 97403 usa. Spliceacceptor site mutation in p53 gene of hu888 zebrafish line.
Pdf detection of the p53 response in zebrafish embryos. The hrpconjugated antirabbit igg antibody gtx211001 was used to detect the primary antibody. In response to cytokines and growth factors, stat family members are phosphorylated by the receptor. Detection of the p53 response in zebrafish embryos using new. Activation of p53 leads to induction of dna repair, cellcycle arrest, apoptosis and senescence. Jmse free fulltext toxicity of deltamethrin to zebrafish. Analyses of p53 target genes in the human genome by.
Furthermore, compared to wildtype siblings, heterozygous stac fish develop invasive tumors at a. Recombinant full length protein corresponding to zebrafish p53. In this system the p53 gene is identified as a p53responsive gene itself. Detection of phosphop53 and p53 detection in ncih460 cells post hydroxyurea treatment hydroxyurea is an inducer of p53 upregulation and phosphorylation as hydroxyurea concentration. In this book, sue armstrong wishes to inform you about p53, a key tumorsuppressor gene or more grandly named as the the guardian of the genome, by telling you stories of the scientists who tried to understand it.
Due to their striking genomic similarity to humans, regenerative abilities. Through the personal accounts of key researchers, the book reveals the excitement of the hunt for new curesthe hype, the enthusiasm, the lost opportunities, the blind alleys and the thrilling breakthroughs. P53 is a reported alias name for the human gene tp53, or tumor protein p53. Fulllength zebrafish p53 is 48% identical in amino acid sequence to human p53 cheng et al. Our results demonstrate that zebrafish p53 protein can readily be induced in embryos and detected using these new antibody. In response to cytokines and growth factors, stat family members are phosphorylated by the receptor associated kinases, and then form homo or heterodimers that translocate to the cell nucleus where they act as transcription activators. Zebrafish models of p53 functions pubmed central pmc. Puma, a novel proapoptotic gene, is induced by p53. Furthermore, compared to wildtype siblings, heterozygous stac fish develop invasive tumors at a dramatically reduced rate, suggesting a reduced cancer risk. Deltamethrin is responsible for health and reproduction problems both in mammals and aquatic organisms.
Genetic screen for lifraumeni syndrome in zebrafish. Introduction p53 is a tumor suppressor gene expressed in a wide variety of tissue types and is involved in regulating cell growth, replication, and apoptosis. Activation of p53 can lead to either cell cycle arrest and dna repair or. Authors conclude that quantitative measurements of intratumor heterogeneity by multiplex fish.
The zebrafish information network zfin is the database of genetic and genomic data for the zebrafish danio rerio as a model organism. Mutational inactivation of the p53 gene product is one of the most common genetic changes seen in human cancers. Detection of the p53 response in zebrafish embryos using. Chk 2 and chk 1 c an phos phory lat e p53 at s er20, enhanc ing it s t et rameriz at ion, s t abilit y, and ac t iv it y 8, 9. Chromatin immunoprecipitations were performed with crosslinked chromatin from hct116 cells. In this study, zebrafish adults were exposed for 15 days to 0. Immunoprecipitation and western blot of p53 protein in hct116 lysates treated with 30um cisplatin, 24 hours using a input, 20 g of hct116 lysates b normal rabbit igg or c 2. Native to south asia, it is a popular aquarium fish, frequently sold under the trade name zebra danio and thus often called a tropical fish. P hos phory lat ion of p53 at s er46 regulat es t he abilit y of p53 t o induc e apopt os is 16. Zfin provides a wide array of expertly curated, organized and crossreferenced zebrafish research data. An important tumor suppressor, the protein mediates cellular growth and proliferation, regulating proteins involved in the stressresponse. This antibody gave a positive signal in roscovitinetreated zebrafish embryo lysates. I nhibit ion of deac et y lat ion s uppres s ing mdm2 f rom rec ruit ing hda c1 c omplex by p19 a rf s t abiliz es p53. Overexpression of wildtype p53 protein in p53 deficient cells can arrest cell proliferation, reverse a tumorigenic phenotype, and sometimes induce apoptosis or differentiation 1, 2.
It is aimed to help labome visitors find the most suited p53 antibody. Chromatin immunoprecipitations were performed with crosslinked chromatin from hct116 cells treated with uv 100 jm 2 followed by a 3 hour recovery and either p53 1c12 mouse mab or normal rabbit. P53 antibody rabbit polyclonal from proteintech validated in western blot wb, immunoprecipitation ip, immunofluorescence if, coimmunoprecipitation ip, chimmunoprecipitation ip, enzymelinked. The data collected includes not only links to publications in. Based on the observation made above, we designed an f3 recessive genetic screen in zebrafish to identify components of the lfsp53. Although p53 plays a critical role in cell cycle control and the regulation of apoptosis, it is largely dispensable for normal embryonic development in fish and mice. Detection of phosphop53 and p53 detection in ncih460 cells post hydroxyurea treatment hydroxyurea is an inducer of p53 upregulation and phosphorylation as hydroxyurea concentration increases, we observe an increase in p53 and phosphop53 signal, and this is depicted in both the image and graphical data above. Chk 2 and chk 1 c an phos phory lat e p53 at s er20, enhanc ing it s t et rameriz at ion, s t abilit y. Antimutant p53 antibody y5 bsa and azide free ab219731 specific references 6 description. Zebrafish p53 is highly similar to mammalian p53 in both structure and function.
Nov 20, 2014 in this book, sue armstrong wishes to inform you about p53, a key tumorsuppressor gene or more grandly named as the the guardian of the genome, by telling you stories of the scientists who tried to understand it. In addition to differences in development, p53null zebrafish embryos also showed. From abcams oem supplier st johns laboratory, validated in wb, elisa. Retinal precursor cells mutant for caf1b arrest in s phase and undergo p53 mediated apoptosis. Tp53 antibody mouse monoclonal p53 antibody prospec.
The availability of antibodies against zebrafish p53 protein would, therefore, offer a prospect of a powerful system for identification of novel. Tumor protein p53, also known as p53, cellular tumor antigen p53 uniprot name, the guardian of the genome, phosphoprotein p53, tumor suppressor p53, antigen nyco, or transformationrelated protein 53 trp53, is any isoform of a protein encoded by homologous genes in various organisms, such as tp53 humans and trp53 mice. We appreciate as much detail as possible and references as appropriate. Antibody ab32049 antimutant p53 antibody y5 by abcam.
1288 658 1180 142 1164 772 1409 1270 1031 206 1282 1513 174 224 502 954 662 1081 1541 76 1189 848 267 843 260 511 2 1559 718 1211 1253 688 832 349 1305 443 782 1049 518 900 1341